Pool Chemistry. Jason Wentworth, C.J Whitaker. The goal of this lab was to explore chlorine’s ability to hinder bacterial growth when it comes to maintaining public pools. Chlorine is used in public pools because it’s not too acidic but it is still very effective at killing bacteria when at appropriate pH levels. Pool standards indicate that chlorine is most effective at pH 7.4-7.8. Tests were performed to determine chlorine’s effectiveness at different p levels. This was done by growing bacteria cultures on agar gel, and then adding pieces of paper soaked in chlorine solutions at different pH levels. It was found that chlorine was most effective at a pH level of 7.4-7.8. These results indicate that chlorine is in fact most effective in a slightly alkaline solution of pH 7.4-7.8.
Key words: Pool chemistry, Pool cleaning, chlorine, pH
Sources: How To Grow Bacteria in Agar Petri Dishes. (n.d.). The Science Company lab supplies, chemicals, microscopes and weather instruments.. Retrieved March 1, 2011, from http://secure.sciencecompany. com/How-To-Grow-Bacteria-in- Agar-Petri-Dishes-W54.aspx: This document summarizes the basics of plate studies. First an agar is needed. To get agar ready heat until a boil, then pour into plates and let sit and dry. After the agar is a gel and not a liquid, streak the bacteria over the agar by sanatising the loop and carfully and evenly streak over the agar. Then place a small, circular piece of paper over a streak that had been dipped in the anit-bacterial solution. After put into incubator to let the bacteria grow. See if there is a zone of inhibition or not.
This site showed us the pool standard levels of pH and chlorine, and how different levels of pH effect bacteria growth. The recommended pH levels for pools is between 7.4-7.8.
Abstract:
Key words: Pool chemistry, Pool cleaning, chlorine, pH
Apparatuses:
pH Meter-
Chloride Ion-Selective Electrode-
Plate Studies Pictures-
Sources:
How To Grow Bacteria in Agar Petri Dishes. (n.d.). The Science Company lab supplies, chemicals, microscopes and weather instruments.. Retrieved March 1, 2011, from http://secure.sciencecompany. com/How-To-Grow-Bacteria-in- Agar-Petri-Dishes-W54.aspx:
This document summarizes the basics of plate studies. First an agar is needed. To get agar ready heat until a boil, then pour into plates and let sit and dry. After the agar is a gel and not a liquid, streak the bacteria over the agar by sanatising the loop and carfully and evenly streak over the agar. Then place a small, circular piece of paper over a streak that had been dipped in the anit-bacterial solution. After put into incubator to let the bacteria grow. See if there is a zone of inhibition or not.
Pool Water Chemistry. (n.d.). Division of Environmental Health - Home. Retrieved March 1, 2011, from http://www.deh.enr.state.nc.us/ehs/quality/wph.html
This site showed us the pool standard levels of pH and chlorine, and how different levels of pH effect bacteria growth. The recommended pH levels for pools is between 7.4-7.8.